Restriction endonuclease analysis using HhaI and HpaII to discriminate among group B Pasteurella multocida associated with haemorrhagic septicaemia
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چکیده
منابع مشابه
Restriction Endonuclease Analysis of Pasteurella Multocida
In this study the restriction endonuclease analysis (REA) technique was used for the comparison of field isolates and vaccinal strain of Pasteurella multocida using Hha-I enzyme. On the basis of DNA finger prints, extent of homology among the field isolates and the vaccinal strain was observed. The study has provided the clear evidence that eight isolates of P. multocida used were serologically...
متن کاملComparative Genomic Analysis of Asian Haemorrhagic Septicaemia-Associated Strains of Pasteurella multocida Identifies More than 90 Haemorrhagic Septicaemia-Specific Genes
Pasteurella multocida is the primary causative agent of a range of economically important diseases in animals, including haemorrhagic septicaemia (HS), a rapidly fatal disease of ungulates. There is limited information available on the diversity of P. multocida strains that cause HS. Therefore, we determined draft genome sequences of ten disease-causing isolates and two vaccine strains and comp...
متن کاملAnalysis of Pasteurella Multocida Strains Isolated from Cattle with Haemorrhagic Septicaemia by the Use of Pfge
Forty Pasteurella multocida subsp. multocida strains collected from cattle with haemorrhagic septicaemia belonging to serotype B:2, 5 and two reference strains of serotype B:2, were examined. The strains were isolated in the years 1958 to 1962 and 1985 to 1995. Analysis of the polimorfism of restricted DNA fragments by the use of the pulsed-field gel electrophoresis (PFGE) technique of contour-...
متن کاملNew sites of localisation of Pasteurella multocida B:2 in buffalo surviving experimental haemorrhagic septicaemia
BACKGROUND Haemorrhagic septicaemia (HS) is an acute septicaemic disease of buffalo and cattle caused by Pasteurella multocida B:2 and E:2. Field outbreaks of HS are known to result in localisation of bacteria in the tonsils of surviving buffalo, confirming that animals can become carriers and the role of respiratory tract in the transmission of the disease. This report describes additional sit...
متن کاملPasteurella multocida P52 aroA mutant conferred protection to rabbits and mice against haemorrhagic septicaemia
Pasteurella multocida serotype B:2 is the etiological agent of haemorrhagic septicaemia (HS) in cattle and buffaloes, a disease that causes significant economic losses to the dairy industry in India. Several killed vaccines are in uses that provide protection to limited period of time. The aim of this study was to generate an attenuated strain of Pasteurella multocida P52 by targeted gene knock...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 2000
ISSN: 0022-2615,1473-5644
DOI: 10.1099/0022-1317-49-1-81